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紫外分光光度法测定长白楤木根、茎、叶中总黄酮含量

Determination of the Total Flavonoids in Roots,Stalks and Leaves of Aralia continentalis Kitagwa by Spectrophotometry

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【作者】 宁德利阮洪生韩承伟黄金仁殷奎德

【Author】 NING De-li1,RUAN Hong-sheng1,HAN Cheng-wei2,HUANG Jin-ren1,YIN Kui-de1*(1.Life Science and Technology College,HLJ August First Land Reclamation University,Daqing,Heilongjiang,163319,China;2.HLJ Forest Vice-special Product Research Institute,Mudanjiang,Heilongjiang 157011,China)

【机构】 黑龙江八一农垦大学生命学院黑龙江省林副特产研究所

【摘要】 目的对长白楤木根、茎、叶中所含总黄酮的含量进行测定。方法采用紫外分光光度法于360 nm波长处测定。结果总黄酮在4.08~32.64μg/m l范围内线性关系良好,其回归方程为:A=0.018 3C+0.012 7,R2=0.999 2;平均加样回收率为100.63%(RSD=1.93%)。结论该方法简便、准确,可用于长白楤木的质量控制。

【Abstract】 Objective To establish a method for the determination of the total flavonoids in roots,stalks and leaves of Aralia continentalis Kitagwa.Methods The total flavonoids in roots,stalks and leaves of Aralia continentalis Kitagwa were determined by spectrophotometric method at 360 nm wavelength.Results Sample size showed a good linear relationship at the range of 4.08~32.64 μg/ml,and the curve was A=0.018 3C+0.012 7,R2=0.999 2.The average recovery was 96.2% and RSD was 1.42%(n=6).Conclusion This method is easy and accurate,and can be used for quality control of Aralia continentalis Kitagwa.

【基金】 黑龙江省农垦总局项目(No.HNKXIV-06-06-04)
  • 【文献出处】 时珍国医国药 ,Lishizhen Medicine and Materia Medica Research , 编辑部邮箱 ,2009年01期
  • 【分类号】R284.2
  • 【被引频次】14
  • 【下载频次】442
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