【摘要】 目的研究microRNA-206在人横纹肌肉瘤细胞A673和A204中的表达以及对细胞增殖的影响。方法Northern blotting检测正常肌肉组织与人横纹肌肉瘤细胞中microRNA-206表达水平的差异,MTS法检测转染microRNA-206后对细胞增殖的影响。Western blotting检测microRNA-206后对细胞中蛋白水平的影响。结果Northern blotting结果显示,microRNA-206在正常肌肉组织中高表达,在横纹肌肉瘤细胞A673和A204中不表达;MTS结果表明转染microRNA-206后,横纹肌肉瘤细胞的数目明显减少,microRNA能够明显下调人横纹肌肉瘤细胞中p-ERK的表达。结论microRNA-206通过ERK信号传导通路抑制横纹肌肉瘤细胞的增殖。更多还原

【Abstract】 Objective To investigate the effect of microRNA-206 on the proliferation of human rhabdomyosarcoma cell line A673 and A204.Methods MicroRNA-206 was transfected into human rhabdomyosarcoma A673 and A204 cells.Northern blotting was performed to detect the expression level of microRNA-206.The proliferation of rhabdomyosarcoma cells was examined by MTS.Western blotting was performed to detect the expression level of protein.Results Northern blotting showed that microRNA-206 was highly expressed in human skeletal muscle but not in rhabdomyosarcoma A673 and A204 cells.After transfection of microRNA-206,the proliferation of A673 and A206 cells was inhibited and the expression of p-ERK was downregulated.Conclusion microRNA-206 can suppress the proliferation of rhabdomyosarcoma cells by downregulation of p-ERK.更多还原