【摘要】 目的:探讨CD46分子对CD4+T细胞的免疫调控作用及其机制。方法:分离人CD4+T淋巴细胞,体外检测CD3、ConA、CD3/CD28、CD3/CD46、CD3/CD28/CD46共刺激对CD4+T细胞的作用效果,并检测其细胞上清液中白细胞介素-2(IL-2)、γ-干扰素(IFN-γ)、白细胞介素-10(IL-10)和转化生长因子-β(TGF-β)的水平。结果:与CD3组或阴性对照组相比,ConA、CD3/CD28、CD3/CD46和CD3/CD28/CD46共刺激后,CD4+T细胞均出现显著增殖反应(P<0.05),而且CD3/CD28/CD46共刺激组的增殖活性较CD3/CD28或CD3/CD46共刺激组显著增高(P<0.05)。CD3/CD28共刺激后,IL-2和IFN-γ水平较阴性对照组和CD3组显著升高(P<0.05),CD3/CD46共刺激后,IL-10和TGF-β水平较阴性对照组、ConA组、CD3组和CD3/CD28共刺激组显著升高(P<0.05)。结论:补体调节蛋白CD46可以诱导CD4+T细胞的增殖反应并产生IL-10和TGF-β,有可能抑制同种移植免疫反应。更多还原

【Abstract】 Objective To investigate the immunoregulation of complement regulatory protein CD46 on CD4+ T cells and its mechanism. Methods CD4 + T cells were isolated from human peripheral blood T lymphocytes by magnetic-activated cell sorting. Then the effect of CD3, ConA, CD3/CD28, CD3/CD46, and CD3/CD28/CD46 costimulation on CD4+ T cells proliferation and levels of interleukin-2 (IL-2),γ-interferon (IFN-γ), interleukin-10 (IL-10), and transforming growth factor-β (TGF-β) in supernatant fluid were detected. Results As compared with CD3 group or negative control group, ConA, CD3 / CD28, CD3/CD46, and CD3/CD28/CD46 costimualtion can induce significant proliferation of CD4+ T cells (P<0.05), especially in CD3/CD28/CD46 costimualtion group (vs CD3/CD28 or CD3/CD46 consitmulation groups, P<0.05). Levels of IL-2 and IFN-γ were significantly increased in CD3/CD28 costimulation group than those in CD3 group and negative control group (P<0.05). Levels of IL-10 and TGF-β were significant increased in CD3/CD46 costimulation group than those in negative control, ConA, CD3, and CD3/CD28 groups (P<0.05). Conclusion Complement regulatory protein CD46 can induce CD4 + T cells proliferation and increase IL-10 and TGF-β, and may inhibit allograft immune response.更多还原