【摘要】 目的探讨新西兰兔脑组织局灶性缺血后再灌注实验动物模型的制作,研究局灶性脑缺血再灌注后新西兰兔脑组织缺氧诱导因子-1α的表达及意义。方法制备兔局灶性脑缺血3h再灌注6h、12h、1d、2d、3d、4d及对照组动物模型,通过免疫组化检测缺氧诱导因子-1(HIF-1)α蛋白的表达,RT-PCR检测HIF-1α基因表达的变化。结果免疫组化结果显示新西兰兔在缺血后再灌注6h开始,缺血侧脑组织梗死灶周边HIF-1α蛋白开始呈阳性表达,随再灌注时间的延长阳性表达进一步增强,一直持续到第3d,第4d开始下降;RT-PCR检测HIF-1α在6h后已有表达,随再灌注时间的延长,HIF-1α的表达呈上升的趋势,在第3d达高峰,第4d显著下降。结论脑缺血再灌注损伤可以诱导HIF-1α表达增强,提示HIF-1α对缺血性脑损伤有神经保护作用。更多还原

【Abstract】 Objective To study the significance and expression of hypoxia inducible factor-1 alpha (HIF-1α) after focal cerebral ischemia reperfusion in New Zealand rabbit experimental models. Methods Divide the animal into 7 groups with random,prepare the focal ischemia experimental model for 3h and reperfuse for 6h,12h,1d,2d,3d,and 4d,then,detect the expression products of HIF-1α in the brain tissues by immunohistochemisty and RT-PCR. Results HIF-1α protein and mRNA began to express after 6h reperfusion,increased significantly on the first and the second days,reached the peak on the third day,then declined significantly on the fourth day through the way of immunohistochemisty and RT-PCR. Conclusions Ischemia-reperfusion injury can strengthen the expression of HIF-1α,which suggests that HIF-1α has a neuroprotective effect on cerebral ischemic injury.更多还原