【摘要】 目的研究(±)2-(7,8,3′,4′,5′-五甲氧基)黄烷(PMF)体外对人胃癌SGC-7901细胞的增殖抑制作用及机制。方法MTT法检测不同浓度PMF体外对SGC-7901细胞的增殖抑制作用;流式细胞仪检测PMF对细胞周期分布的影响;Western blot法检测PMF对凋亡相关蛋白PARP、caspase-3表达的影响。结果不同浓度的PMF作用72 h可剂量依赖性地抑制SGC-7901细胞增殖;PMF作用12 h可使SGC-7901细胞周期阻滞于G2/M期;PMF作用24 h细胞周期检测可见亚二倍体峰(SubG1),并可诱导细胞凋亡相关蛋白PARP、caspase-3的活化。结论PMF体外可抑制人胃癌SGC-7901细胞的增殖,其增殖抑制作用与诱导G2/M周期阻滞和细胞凋亡有关。更多还原

【Abstract】 Objective To investigate the growth inhibitory effect and mechanism of(±)2-(7,8,3′,4′,5′-pentamethoxyflavan)(PMF) on human gastric cancer SGC-7901 cells.Methods SGC-7901 cells were treated with different concentration of PMF.The growth inhibition was measured by MTT assay;the change of cel1 cycle distribution was determined by flow cytometry analysis;the activation of PARP and caspase-3 was probed using Western blot assay.Results After treatment with PMF for 12 h,the growth of SGC-7901 cells were significantly inhibited in a dose-dependent manner;meanwhile,the cell cycle SGC-7901 was arrested at G2/M phase.Moreover,the sub-G1 peak was detected by flow cytometry analysis,and the activation of PARP and caspase-3 was observed in SGC-7901 cells treated with PMF for 24 h.Conclusions PMF inhibits the growth of SGC-7901 cells through arrest of G2/M and induction of apoptosis.更多还原