【摘要】 目的研究99mTc标记抗人胰腺癌单克隆抗体SZ-121在荷人胰腺癌裸鼠体内的显像及生物分布,探讨其用于胰腺癌早期诊断的可行性。方法采用皮下种植法建立荷人胰腺癌裸鼠移植瘤模型,以2-亚氨基噻吩盐酸盐(2-IT)修饰SZ-121,99mTc-葡庚糖酸钠(GH)配体交换法标记SZ-121,经裸鼠尾静脉注入99mTc-SZ-121后1、4h对裸鼠进行γ显像,于24h测定荷瘤小鼠体内组织的放射性分布。结果γ显像及生物分布显示,99mTc-SZ-121被静脉注入荷瘤鼠后1h,肿瘤即清晰显影,随时间延长趋于增浓,且瘤组织与非瘤组织的放射性比值(T/NT)也逐渐增高,肿瘤每克组织百分注射剂量率(%IDPg)均高于注射99mTc-IgG的对照组(P<0.05)。结论99mTc-SZ-121具有活体内定位导向能力,显像时间短,可能是一种有前途的胰腺癌显像剂。更多还原

【Abstract】 Objective To study the image and biodistribution of 99mTc labeled anti-pancreatic cancer monoclonal antibody(McAb) SZ-121 in human pancreas carcinoma xenografts,and to develop new technology for early diagnosis of pancreas carcinoma.Methods The McAb SZ-121 was labeled with 99mTc using 2-iminothiolane modification and 99mTc-glucoheptonate(GH) transchelation method.The images were taken using γ scintigraphy 1,4 h after injection.and the distribution 24 h after injection of 99mTc-SZ-121 was observed.Results Clear tumor image was obtained 1 h after injection of 99mTc-SZ-121.The radioactivity in the tumor and the radioactivity ratio of tumor to non-tumor tissue increased as time passing by.The %IDPg of tumor tissue in experiment group was higher than that in the control group at the 24 th h(P<0.05).Conclusion 99mTc-SZ-121 has a capacity of in vivo oriented positioning,imaing time is short.So it perhaps is a promising pancreas carcinoma imaging agent.更多还原