【摘要】 目的研究体外香烟烟雾对V79细胞增殖和凋亡的影响。方法将V79细胞分为对照组、5%浓度组和20%浓度组进行染烟实验。染烟后检测细胞内ROS水平、细胞增殖活性、细胞周期及凋亡情况。结果5%浓度和20%浓度染烟组与对照组相比,V79细胞ROS水平均明显升高,且20%浓度组与5%浓度组相比ROS也明显升高,差异均有统计学意义(均P<0.05)。5%浓度和20%浓度染烟组与对照组相比,细胞增殖率、克隆率明显降低;G1期细胞百分比明显升高,G2期和S期细胞明显减少,差异均有统计学意义(均P<0.05)。20%浓度染烟组分别与对照组和5%浓度染烟组相比,细胞凋亡率均明显升高,差异有统计学意义(P<0.05)。结论香烟烟雾可能刺激了V79细胞内ROS的增多,影响细胞增殖和凋亡率。更多还原

【Abstract】 Objective To investigate the effects of tobacco smoke on proliferation and apoptosis of V79 cells.Methods V79 cells were divided into three groups:the control group,5% group,20% group.The level of ROS,the effect of cell growth inhibition,the cell cycle and apoptosis were assayed.Results The level of ROS in the 5% and 20% groups was significantly higher than that in the control group,and ROS in the 20% group was significantly higher than that in the 5% group(P<0.05);the cell growth rate and cloning rate in the 5% and 20% groups were significantly lower than that in the control group(P<0.05),the percentage of cells in G1 phase in the 5% and 20% groups was significantly higher,while G2 those in phase and S phase were significantly lower than that in the control group.The apoptoticrates in the 20% group was significantly higher than those in the control group and the 5% group(P< 0.05).Conclusion Tobacco smoke may effet proliferation and apoptoticrates of V79 cells via stimulating the production of ROS in cells.更多还原