【摘要】 目的研究梗阻性黄疸(obstructive jaundice,OJ)幼鼠肠黏膜细胞免疫功能的变化和能全素(Nutrison)喂养对实验鼠肠黏膜免疫功能的保护作用。方法将普通级雄性Wistar大鼠40只,随机分为对照组、假手术组、OJ组、OJ+能全素(OJ+N)组,每组10只。流式细胞术检测大鼠肠黏膜CD4+、CD8+淋巴细胞,并计算CD4+/CD8+的比值。结果OJ组大鼠肠黏膜CD4+淋巴细胞百分数与对照组、假手术组和OJ+N组比较差异有统计学意义(P<0.05),OJ+N组CD4+/CD8+比值较OJ组显著升高,OJ组CD4+/CD8+比值明显低于对照组和假手术组(P均<0.05)。结论OJ显著降低大鼠肠黏膜CD4+淋巴细胞百分数,OJ+N组与OJ组比较,CD4+淋巴细胞百分比显著增加。OJ组肠黏膜CD4+/CD8+比值较对照组和假手术组及OJ+N组显著降低,肠内营养对肠黏膜免疫功能有保护作用,为临床上应用肠内营养改善梗阻性黄疸患者的肠黏膜局部免疫功能提供了初步的佐证。更多还原

【Abstract】 Objective To investigate the immune defense and the effects of enteral nutrition on the impaired immune defense in an obstructive jaundice model of rats.Methods 40 male Wistar rats were randomly divided into 4 groups: control,sham operation,OJ,OJ+Nutrison.The number of intestinal mucosal CD4+,CD8+ T lymphocytes were detected by flow cytometry technique.Results A significant decrease in numbers of intestinal mucosa CD4+ T lymphocytes was seen in OJ and OJ+N group,when compared with control and sham group,P<0.01,respectively.The ratio of CD4+/CD8+ T lymphocytes in OJ group decreased significantly in comparison with the other three groups(P<0.05).Conclusion It was suggested that OJ affects the intestinal mucosal immunity and enteral nutrition can improve damaged intestinal mucosal cellular immunity.更多还原