【摘要】 目的探讨人直肠癌中环氧化酶-2(COX-2)、基质金属蛋白酶-2(MMP-2)的形态表达特点及其与侵袭和转移的关系。方法选取直肠癌组织46例,正常直肠组织12例,分别应用COX-2单克隆抗体和MMP-2单克隆抗体进行免疫组织化学染色,观察COX-2、MMP-2的表达水平,结果进行统计学处理。结果COX-2在直肠癌中的阳性表达率为63.04%,在正常直肠组织中阳性表达率为25%,差别有统计学意义(P<0.05);其表达程度与直肠癌的临床分期和转移有关(P<0.05),而与其组织学类型及分级无关(P>0.05)。MMP-2在直肠癌中的表达率为67.39%,在正常直肠组织中表达率为25%,差异有统计学意义(P<0.05);其表达程度与直肠癌的分级、临床分期和转移有关(P<0.05),与其组织学类型无关(P>0.05)。结论COX-2、MMP-2在直肠癌组织中均呈过表达,其表达程度具有相关性。COX-2、MMP-2的表达与直肠癌的发生、侵袭和转移密切相关。更多还原

【Abstract】 Objective To study the expression of cyclooxygenase-2(COX-2) and matrix metalloproteinase-2(MMP-2) in rectal carcinoma and their relationship with tumor cell invasion and metastasis.MethodsUsing monoclonal antibodies for COX-2 and MMP-2,the expression of COX-2 and MMP-2 were detected by means of immunohistochemistry in 46 cases of rectal carcinoma and 12 cases normal rectal tissue as controls.The results were analysis statistically.ResultsThe positive expression rate of COX-2 and MMP-2 in rectal carcinoma and normal rectal tissues was 63.04% and 25%(P<0.05),67.39% and 25%(P<0.05),respectively.The expression of COX-2 was intimately related to Dukes stage and metastasis of rectal carcinoma(P<0.05),but not related to subset of cells and pathological grade of rectal carcinoma(P>0.05);while MMP-2 was closely related to pathological grade,Dukes stage and metastasis of rectal carcinoma(P<0.05),not related to subset of cells of rectal carcinoma(P>0.05).ConclusionCOX-2 and MMP-2 are over-expressed in rectal carcinoma with certain correlation.The over-expression of COX-2 and MMP-2 was significantly correlated to its invasion and metastasis and metastasis.更多还原