【摘要】 目的研究凋亡抑制蛋白Survivin和核转录因子(NF-κB)在大肠黏膜癌变过程中的表达,初步探讨两者在大肠癌发生、发展中的作用及其与大肠癌临床病理特征的关系。方法采用免疫组织化学SP法检测67例大肠腺癌、51例大肠腺瘤(包括26例低度异型增生和25例高度异型增生)和22例正常大肠黏膜组织中Survivin和NF-κB的表达。结果Survivin和NF-κB在大肠黏膜癌变过程中的表达率逐渐升高,Survivin在大肠正常黏膜、低度异型增生腺瘤、高度异型增生腺瘤、大肠癌中阳性表达率分别为9.1%(2/22)、23.1%(6/26)、52.0%(13/25)和58.2%(39/67);NF-κB在大肠正常黏膜、低度异型增生腺瘤、高度异型增生腺瘤、大肠癌中阳性表达率分别为13.6%(3/22)、26.9%(7/26)、64.0%(16/25)和67.2%(45/67)。Survivn、NF-κB的表达与大肠癌的侵袭深度和淋巴结转移及Dukes分期有关,与性别、年龄无关;Survivn、NF-κB在大肠癌中的表达具有相关性(χ2=6.363,P=0.012)。结论Survivn、NF-κB在大肠黏膜癌变过程及大肠癌的浸润、转移中起重要作用,两者共同促进了大肠癌的发生、发展。更多还原

【Abstract】 Objective To investigate the expressions of survivin and NF-κB in colorectal cancer,analyze the role of survivin and NF-κB in carcinogenesis of colorectal cancer and define the correlation of survivin and NF-κB expressions with clinicopathologic features.Methods We used SP immunohistochemistry method to detect the expressions of survivin and NF-κB in 67 cases of colorectal cancer,51 adenomas(including 26 colorectal low-grade atypical hyperplasia and 25 high-grade atypical hyperplasia)and 22 normal mucosa.Results The expressions of survivin and NF-κB gradually increased in carcinogenesis of colorectal mucosa.The expressions of survivin in normal mucosa,low-grade atypical hyperplasia,high-grade atypical hyperplasia and colorectal cancer were 9.1%(2/22),23.1%(6/26),52.0%(13/25)and 58.2%(39/67)respectively;and the expressions of NF-κB were 13.6%(3/22),26.9%(7/26),64.0%(16/25)and 67.2%(45/67)respectively.It was revealed that the expressions of survivin and NF-κB correlated with lymph node metastasis,the depth of invasion(P <0.05)and the Duke,s stage of the tumor.In addition,it was found that there was a significant correlation between the expression of survivin and the expression of NF-κB(P <0.05)in colorectal cancer.Conclusion The results suggest that survivin and NF-κB may play an important role in colorectal carcinogenesis and malignant progression of colorectal cancer.更多还原