【摘要】 目的:观察低剂量蟾蜍灵、全反式维甲酸(All-trans retinoic acid,ATRA)单药或联合应用对HL-60细胞增殖及分化的影响,同时检测上游通路Syk表达的变化。方法:流式细胞仪技术检测CD11b表达;West-ern Blot检测Syk和Tubulin表达。结果:与对照组相比,低剂量蟾蜍灵、ATRA或联合用药不同程度地抑制HL-60细胞增殖,同时蟾蜍灵联合ATRA以时间依赖性的方式诱导HL-60细胞分化,并伴有Syk表达上调,但是Syk的酪氨酸磷酸化并未受影响。结论:Syk表达上调可能参与调节低剂量蟾蜍灵联合ATRA对HL-60细胞的分化诱导作用。更多还原

【Abstract】 Objective:To investigate the changes of proliferation and differentiation,the changes of spleen protein tyrosine kinase Syk during HL-60 cells differentiation induced by low dose of bufalin and ATRA.Mehtods:The expression of CD11b was detected using flow cytometery assay.The expressions of Syk and Tubulin were tested using Western Blot.Results:Cells treated with bufalin,ATRA and the combination of bufalin and ATRA showed strong growth inhibition,compared to the control cells.Furthermore the combination of 5 nmol/L bufalin and 30 nmol/L ATRA induced HL-60 cells differentiation in a time-dependent manner.Meanwhile the expression of Syk was also up-regulated in a time-dependent manner,but the tyrosine phosphorylation of Syk was not affected.Conclusion:Up-regulation of Syk might be involved in the regulation of HL-60 cells differentiation.更多还原