【摘要】 目的:检测NOK蛋白在非小细胞肺癌中的表达,分析其表达率与病理分型、分级及临床TNM分期间的关系,为研究NOK的功能及其机制提供临床证据。方法:采用免疫组织化学EnVinsion法检测NOK蛋白在非小细胞肺癌(NSCLC)中的表达。结果:免疫组化结果显示NOK阳性表达主要位于胞质中。NSCLC中NOK总阳性率68.1%,癌旁组织阳性率只有12.1%,差异性非常显著(P=0.000);肺鳞癌和腺癌总阳性率分别为60.49%、78.33%,两者差异显著(P=0.009);鳞癌中分化、低分化阳性率分别为51.1%、72.22%,差异显著(P=0.01),腺癌的高、中、低分级的阳性率分别是20.0%、79.5%、100%,经Kruskal-WallisH检验差异性非常显著(P=0.001);NSCLC(鳞癌、腺癌)TNM分期Ⅱa、Ⅱb、Ⅲa、Ⅲb的阳性率,经Kruskal-WallisH检验其差异性非常显著(P=0.000)。结论:NOK在肺鳞癌、腺癌中高表达,表达的高低与病理分型、分级及临床TNM分期(转移)有关。更多还原

【Abstract】 Objective:To investigate the expression of NOK protein,and analyze the relationship between NOK expression and pathological types,grades and clinical stages(TNM stages) in the non small cell lung cancer(NSCLC).Methods:Samples from 141 cases with NSCLC were collected to detect the expression of NOK protein by immunohistochemical staining(EnVinsion).Results:The NOK positive expression was mainly located in the cytoplasm.NOK positive staining was 68.1% in NSCLC,while the positive rate of the same samples adjacent tissues was 12.1%(P=0.000).Samples with NOK positive staining were observed to cover 60.49% and 78.33% in the squamous cell lung cancers and the lung adenocarcinomas respectively(P=0.009).The positive NOK staining were 51.1% and 72.22% in the moderately differentiated and low differentiated squamous cell lung cancers respectively(P=0.01),while the positive staining covers 20.0%,79.5%,and 100% in the highly,moderately and low differentiated lung adenocarcinoma respectively(P=0.001).Significant differences of the NOK positive staining among different TNM stages in NSCLC were also observed by Kruskal-Wallis test(P=0.000).Conclusion:The NOK protein level is elevated in NSCLC,and the expression is correlated with the pathological type,grade and TNM stage in NSCLC.更多还原