【摘要】 目的:探讨微型染色体维持蛋白(minichromosome maintenance proteins,MCMs)家族中的MCM2蛋白在乳腺癌中的表达及其意义。方法:采用免疫组化染色方法,检测10例正常乳腺上皮、15例乳腺良性肿瘤及60例乳腺癌组织中MCM2蛋白的表达,并以标记指数(labelling index,LI)量化其表达。结果:MCM2在乳腺癌中的表达主要在细胞核,间质细胞亦有少量阳性着色。MCM2的LI在乳腺癌患者不同年龄之间无显著性差异(P>0.05),但在不同TNM分期和不同病理组织学分级(Ⅰ、Ⅱ、Ⅲ)及淋巴结转移之间均有显著性差异,且呈逐渐升高趋势。各组中MCM2标记指数均高于ki-67。MCM2和Ki-67在乳腺癌中的表达呈平行关系(P<0.001)。结论:MCM2是反映细胞增殖的良好指标,作用优于ki-67,其表达指数与乳腺癌的组织分型、细胞增殖及恶性程度有关。MCM2的表达可以提示乳腺癌的恶性程度高低和良性肿瘤的恶变可能性,有助于临床判断患者的预后以及选择治疗方法。更多还原

【Abstract】 Objective:To investigate the expression of MCM2 (a member of MCMs family) in breast cancer and to assess the relationship between MCM2 expression and clinicopathological characteristics of breast cancer. Methods: Immunhistochemical staining was used to detect the expression of MCM2 in 60 patients with breast cancer,and the results were quantified by calculating the labelling index (LI). Results: There was no statistical significance between the LI of MCM2 and some clinicopathological characteristics such as the age. The expression of MCM2 showed a significant association with histological grade、tumor stage and Metastatic status (P<0.05). MCM2 (LI) was higher than Ki-67 LI in each group of breast cancer; The expression of MCM2 and Ki-67 correlated directly with each other(P<0.01). Conclusion: MCM2 may serve as a reliable and useful diagnostic marker for breast cancer and can predict the degree of differentiation in breast cancer ,which may help to predict the clinical prognosis of the patients.更多还原