【摘要】 目的:观察PPARγ在反流物诱导人正常食管上皮细胞增殖变化过程中的表达,及其在ERK信号传导通路中的作用。方法:体外培养人正常食管粘膜上皮细胞,分为酸作用组、胆酸作用组、PPARγ活化组、ERK抑制组及正常对照组。采用MTT法测定细胞增殖状态。细胞免疫化学染色及免疫印迹法测定PPARγ蛋白表达。结果:PPARγ在酸作用组、胆酸作用组及正常对照组呈阴性表达,在PPARγ活化组及ERK抑制组呈阳性表达,细胞免疫化学阳性染色位于细胞核。活化PPARγ可阻断酸、胆酸诱导的细胞增殖。结论:体外培养的正常食管细胞存在PPARγ分布,但在正常情况下及反流物刺激时呈非活性状态。PPARγ可能作为ERK通路的下游因子,参与了反流物诱导正常食管细胞的增殖过程。更多还原

【Abstract】 Objective:To observe the expression of peroxisome proliferators activited receptor γ(PPARγ) in proliferation of normal human esophageal epithelial cells induced by refluxate in vitro,and its role in signal transduction pathway of extracellular signal regulated protein kinase(ERK).Methods:Normal human esophageal epithelial cells cultured in vitro were divided to acid group,bile acid group,PPARγ activation group and ERK inhibition group,in comparison with the control group.Cell proliferation was assessed using MTT.The expression of PPARγ protein was determined by cell immunochemistry and immunoblotting technique.Results:There was negative expression of PPARγ in acid group,bile acid group and control group there was positive expression in PPARγ activation group and ERK inhibition group,with the positive staining in nucleus.Proliferation induced by acid or bile acid exposure was abolished by PPARγ activation.Conclusion:PPARγ exists in normal human esophageal epithelial cells in vitro with the unreactive condition,even after the stimuli of refluxate.PPARγ may play a role in proliferation of normal esophageal cells induced by refluxate as a downstream factor of ERK pathway.更多还原