【摘要】 目的:观察血小板源生长因子(PDGF)受体与细胞外信号调节蛋白激酶(ERK1/2)在再狭窄血管中的表达及川芎嗪(TMP)对其的影响,探讨TMP防治血管再狭窄的作用机制。方法:制备大鼠血管再狭窄模型,采用不同浓度的川芎嗪干预,观察颈总动脉形态学改变,免疫组化法检测颈总动脉PDGF-β受体与ERK1/2的表达。结果:假手术组动脉各层结构正常、清晰,无明显血管平滑肌细胞(VSMC)增殖,颈总动脉损伤大鼠血管平滑肌细胞(VSMC)大量增殖,内膜显著增厚,川芎嗪处理后,血管平滑肌细胞(VSMC)增殖程度降低;与假手术组比较,再狭窄大鼠颈总动脉PDGF-β受体与ERK1/2表达显著增强(P<0.01),中、高剂量川芎嗪明显降低PDGF-β受体及ERK1/2的表达(P<0.01,P<0.05)。结论:TMP抑制血管再狭窄,其机制与抑制血管平滑肌细胞PDGF受体及ERK1/2表达有关。更多还原

【Abstract】 Objective:To study the effects of tetramethylpyrazine(TMP)on vascular restenosis in rats.Methods:Restenosis model was established.Treatment with TMP in different dose.The morphologic change,the expression of PDGF-βreceptor and extracellular signal regulated protein kinase 1/2(ERK1/2)were detected.Results:Compared with sham,the expression of PDGF-βand ERK1/2 were obviously increased in operation group(P<0.01),the VSMCs proliferation was inhibited and expression of PDGF-βand ERK1/2 were decreased obviously by TMP.Conclusion:TMP play an important role in cure restenosis through inhibiting the expression of PDGF-β receptor and ERK1/2.更多还原