【摘要】 目的探讨成年大鼠骨髓基质细胞(BMSCs)在低氧环境中向神经细胞分化的可能性。方法从大鼠骨髓中分离培养BMSCs,联合BMSCs表面标志物CD106及造血干细胞特异标志物CD34对BMSCs进行鉴定,然后将其放入3%低氧环境中培养,观察细胞形态的变化,并采用免疫荧光检测诱导后的细胞是否表达神经元特异性烯醇化酶(NSE)标志物。结果经鉴定,培养的BMSCs细胞CD106阳性,CD34阴性。低氧环境中培养7d,部分细胞的形态已发生改变,14d后大部分细胞不仅在形态上表现为神经元样特征,而且NSE呈阳性表达。结论低氧能将BMSCs诱导分化为神经元样细胞。更多还原

【Abstract】 Objective To investigate the possibility of adult rat bone marrow stromal cells(BMSCs)differentiating into neural cells under hypoxia.Methods BMSCs were isolated and cultured from the bone marrow of rat,and then identified by CD106 and CD34.The identified BMSCs were cultured in 3% oxygen.Then the morphological change of BMSCs was observed,and neuron specific enolase(NSE)was detected by immunofluorescence.Results When cultured for 7 d,forms of some BMSCs had been changed.After 14 d,a majority of BMSCs showed characteristics of neural cells with NSE positive.Conclusion Hypoxia may induce BMSCs differentiating into neuron-like cells.更多还原