【摘要】 目的观测genistein后处理对在体大鼠心脏局部缺血/再灌注模型大鼠心肌细胞凋亡、心肌组织病理形态学及超微结构变化的影响。方法40只实验动物随机分为5组,分别为假手术组、缺血/再灌注组、genistein0.1,1.0,5.0mg/kg后处理给药组。光镜下观察各组心肌组织病理变化,电镜下观察各组心肌组织超微结构变化,DNA原位末端缺口标记法(TUNEL)检测各组心肌细胞凋亡数,观察凋亡指数变化,检测各组心肌组织中caspase-3活性,观察caspase-3的比活性变化。结果各剂量genistein后处理组的凋亡指数与缺血/再灌注组相比均显著下降,各组caspase-3比活性变化趋势与凋亡指数变化趋势相吻合。心肌组织损伤的病理形态学和超微结构随给药剂量不同而变化,呈现出1.0mg/kg组损伤最轻,0.1mg/kg时损伤次之,5.0mg/kg时损伤又较重。结论genistein后处理对在体大鼠心肌缺血/再灌注损伤具有保护作用,保护机制与抑制心肌细胞凋亡有关,并且该保护效应与给药剂量相关。更多还原

【Abstract】 Objective To explore the effect of genistein pharmacological post-treatment on the cardiocyte apoptosis,pathomorphology and ultrastructural changes of myocardial tissue in rats with ischemia-reperfusion injury.Methods Forty rats were randomized into 5 groups:sham group,ischemia-reperfusion group and 0.1,1.0,5.0 mg/kg genistein post-treatment groups.The pathological and the ultrastructural changes of cardiac muscle were observed under light microscope and electron microscope,respectively.The apoptosis index was determined using TUNEL,and the activity of caspase-3 was detected.Results The apoptosis index and the activity of caspase-3 significantly decreased in genistein post-treatment groups compared with ischemia-reperfusion group.In genistein post-treatment groups,the pathomorphology and ultrastructure of myocardium tissue showed the most serious injury in 5.0 mg/kg genistein post-treatment group,and the lightest in 1.0 mg/kg genistein post-treatment group.Conclusion Genistein pharmacological post-treatment has a protective effect on ischemia-reperfusion injury in rats,which may be induced by the inhibition of cardiocyte apoptosis.The protective effect is related to the dosage.更多还原