【摘要】 提出了一种基于微乳液聚合技术共价固定指示剂染料制备荧光纳米颗粒的新工艺。用甲基丙烯酰氯向荧光染料3-氨基-9-乙基咔唑(AEC)分子中引入末端双键,得化合物3-(N-甲基丙烯酰基)氨基-9-乙基咔唑(MAEC),通过微乳液聚合技术与甲基丙烯酸丁酯共聚合,得到聚合物颗粒,经洗涤、干燥、研磨,得固定AEC的荧光纳米颗粒。该荧光纳米颗粒在使用过程中无染料流失、相对于游离的AEC而言具有更好的耐光漂白性。甲硝唑可猝灭该荧光纳米颗粒的荧光,此性质被用来测定甲硝唑。实验结果显示,在pH 7.3的磷酸盐缓冲溶液/乙醇的混合介质(1/1,体积比)中,荧光纳米颗粒悬浮液(0.30 mg/mL)对甲硝唑有线性响应,线性响应范围为2.0×10-5~1.0×10-3mol/L,检测下限为9.0×10-6mol/L。更多还原

【Abstract】 A novel technique based on covalent immobilization of indicator dye by microemulsion polymerization was developed to prepare fluorescence nanoparticles.A terminal double bond was attached to 3-amino-9-ethylcarbazole(AEC) via methacryloyl chloride.The resulting compound,3-(N-methacryloyl)amino-9-ethylcarbazole(MAEC),was copolymerized with butyl methacrylate by microemulsion polymerization.The polymer particles were formed,washed,dried and ground.The AEC-immobilized fluorescence nanoparticles were obtained,showing no dye-leaching,higher photostability in comparison with free AEC.Metronidazole can quench the fluorescence intensity of the fluorescence nanoparticles.This property was used to assay metronidazole.Experiment results show that a suspension of the fluorescence nanoparticles(0.30 mg/mL) in a mixed medium of pH 7.3 phosphate buffer and ethanol(1/1,volume ratio) has a linear response to metronidazole from 2.0×10-5 mol/L to 1.0×10-3 mol/L.The detection limit is 9.0×10-6 mol/L.更多还原