【摘要】 〔四-[3-甲氧基-4-(N-咔唑)正丁氧苯基],4C4-TPP〕和〔四-[3-甲氧基-4-(N-咔唑)正己氧苯基],4C6-TPP〕是两个结构相似但侧链不同的卟啉化合物,4C6-TPP的侧链长于4C4-TPP。应用紫外吸收光谱、荧光光谱和园二色谱,研究了4C4-TPP和4C6-TPP与小牛胸腺DNA(Calfthymus,ctDNA)之间的相互作用。结果表明:4C4-TPP和4C6-TPP均以侧链插入DNA与之作用,计算二者与DNA之间的结合常数,4C6-TPP与DNA的结合常数远大于4C4-TPP与DNA的结合常数。基于4C4-TPP与4C6-TPP二者之间结构差异仅在于侧链基团,证明了侧链基团对于卟啉与DNA作用的影响不是主要决定于其空间尺寸大小,取代基化学结构是影响卟啉与DNA的相互作用的重要因素。更多还原

【Abstract】 4C4-TPP(meso-tetrakis[3-methoxy-4-(N-carbazyl)n-buxyloxyphenyl]porphyrin) and 4C6-TPP (meso-tetrakis [3-methoxy-4-(N-carbazyl)n-hexyloxyphenyl]porphyrin) are similar in structure,but the substitutent size of later is longer than that of former.The interaction of 4C4-TPP and 4C6-TPP with calf thymus-DNA (ctDNA) was explored by UV-vis,fluorescence and circular dichroism spectra.The results demonstrated that the outside of 4C4-TPP and 4C6-TPP binds ctDNA but peripheral substitutents intercalated.The binding constant of 4C6-TPP with ctDNA is greater than that of 4C4-TPP.The difference of binding between 4C4-TPP and 4C6-TPP suggests that the chemical structure of peripheral substitutent plays a more important role than merely their size in determining the binding preferences of porphyrins.更多还原