【摘要】 目的:探讨乙型肝炎病毒核酸(HBV-DNA)荧光定量聚合酶链反应(FQ-PCR)在样本处理后立即加样扩增和4℃冰箱静置24h后再加样扩增对检测结果的影响。方法:68例不同模式乙肝患者样本分别用2种方法处理后进行HBV-DNAFQ-PCR检测。结果:68例样本采用第1种方法共检出HBV-DNA阳性35例,第2种方法共检出39例;第2种方法HBV-DNA阳性定量结果普遍高于第1种方法,指数平均高1个次方左右(u=5.14,P<0.05);以第2种方法检测结果为依据,则第1种方法有4例阳性结果漏检,检测结果假阴性率达10.3%(4/39)。结论:样本处理后,应置4℃冰箱静置一定时间,以保证病毒颗粒充分裂解后再进行HBV-DNAFQ-PCR,以提高HBV-DNA的检出率。更多还原

【Abstract】 Objective:To investigate the effects of immediate amplication of sample as well as amplication for disposed samples after 24 h standing in 4℃ fridge on HBV-DNA detection results by using FQ-PCR method.Methods:To execute HBV-DNA detection on 68 cases of different modes of HB patients by using FQ-PCR method in two different ways.Results:35 cases with positive HBV-DNA among total 68 cases of samples were detected in the first way,39 cases with positive HBV-DNA were detected in second way.The quantitative result of positive HBV-DNA detected in second way is universally higher than that in the first way,the average index being on square(u=5.14,P<0.05).Based on the second detection result,4 cases with positive HBV-DNA were left out when the first way was adopted,with false negative rate of HBV-DNA being 10.3%(4 /39).Conclusion:After samples of HBV-DNA FQ-PCR have been disposed,they should be put in 4℃ for a certain time of standing to ensure that virus particles fission completely before they were amplified in order to increase the sample detection rate.更多还原