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有机复合物H对纤维素酶促反应动力学特性的影响

Effect of Composite Organic Compounds H on Dynamics Characteristic of the Cellulase Catalyze

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【作者】 李鸿玉厉重先

【Author】 Li Hongyu Li Chongxian(Teacher’s College of Beijing Union University,Beijing 100011)

【机构】 北京联合大学师范学院

【摘要】 采用3,5-二硝基水杨酸(DNS)为显色剂,滤纸和CMC为底物,测定纤维素酶的酶活。考察了在不同温度、pH值、反应时间、底物浓度等反应条件下,有机复合物H对纤维素酶滤纸酶活(FPA)和CMC酶活(CMCA)的影响。结果表明,有机复合物H对纤维素酶的FPA活性和CMCA活性均有一定的促进作用,且在不同条件下的促进效果有较大差异。

【Abstract】 The test of the activity of cellulase was measured with 3,5-dinitrosalicylic acid(DNS)as reagent,filter paper and CMC as the substrate.The effect of composite organic compounds H to FPA and CMC cellulose activity was investigated in different temperature,pH,reaction time and the concentration of the substrate material.The results indicated that composite organic compounds H can distinctly promote the activity of CMC and FPA,and the difference varied according to the conditions.

  • 【文献出处】 生物技术通报 ,Biotechnology Bulletin , 编辑部邮箱 ,2009年12期
  • 【分类号】O621.2
  • 【被引频次】3
  • 【下载频次】63
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