【摘要】 构建饱和的基因突变体库是最直接、有效的分析鉴定基因功能的方法。根据插入突变源不同可分为T-DNA插入突变、转座子插入突变等。主要介绍这两种方法的原理及其在水稻功能基因组学研究中的应用和进展,并分析和讨论了插入突变在水稻功能基因组学研究中存在的困难和发展趋势。更多还原

【Abstract】 Insertional mutagenesis has been known as a reliable method to construct a rice mutant library.According to the sources of insertional mutagenesis,the mutagenesis can be divided into T-DNA insertional mutagenesis,transposon insertion mutation.This paper mainly introduced the principles of these two methods and its application in rice functional genomics research,especially in rice functional genomics study更多还原