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生黑醋酸杆菌在高浓度山梨醇下的半连续发酵特性

Studies on the Characteristic of Gluconbacter melanogenus with High Concentration of D-sordibol in Semi-continuous Fermentation

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【作者】 荆士华汲涌李春杰姜铭妍杨伟超谌明徐慧

【Author】 JING Shi-hua1,2,JI Yong2,LI Chun-jie2,JIANG Min-yan2,YANG Wei-chao1,CHEN Ming2,XU Hui1(1.Institute of Applied Ecology,Chinese Academy of Sciences,Shenyang 110016,China;2.General Pharmaceutical Factory of Northeast,Shenyang 110026,China)

【机构】 中国科学院沈阳应用生态研究所东北制药总厂

【摘要】 目的:调节生黑醋酸杆菌生物和代谢特性,以提高发酵效率。方法:通过改变种液特性,采用半连续培养的方式,对生黑醋酸杆菌在高醇浓度下的生长特性进行了研究。结果:通过优化可以提高VC一步发酵底物山梨醇浓度达38%,32h左右发酵率达95%,山梨糖产量达360mg/ml,半连续培养连续5批之间产糖稳定,没有明显差别。结论:通过优化,有效地提高了山梨糖的产率。

【Abstract】 Objective:To control the characteristic of Gluconbacter melanogenus in medium with high concentration of D-sordibol(38%).Method:by changing seed liquid and using semi-continuous fermentation.Result:The strain could be cultured well in medium containing D-sorbitol in 38%.The conversion rate of D-sorbitol to L-sorbose in medium with 38% D-sorbitol was 95% over 30 hours.There was no notably different about the yields of L-sorbose in 5 generations fermentations continuously.Conclusion: The productivity of sorbose was improved notably.

【关键词】 发酵山梨醇L-山梨糖种液
【Key words】 fermentationsorbitolL-sorboseseed liquid
  • 【分类号】Q93
  • 【被引频次】1
  • 【下载频次】151
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