【摘要】 以芘为惟一碳源,采用富集培养方法,从沈抚灌区石油污染土壤中分离得到一株芘降解菌B05。根据形态学观察、生理生化鉴定和16S rDNA序列分析结果,将菌株B05鉴定为Aminobacter ciceronei。在芘初始浓度为1mg/L的液体无机盐培养基中,培养10d,菌株B05对芘的降解率为51%;在芘初始浓度为1mg/kg的土壤培养基条件下,培养30d,菌株B05对芘的降解率可达51%;在芘初始浓度为50mg/L的乙醇液体培养基条件下,培养5d,菌株B05对芘的降解率可达25.9%。对菌株培养条件进行优化,经SlideWrite统计软件拟合,菌株B05在牛肉膏蛋白胨液体培养基上的最适生长pH值为7.3,最适生长温度为32.5℃,最适装液量为25.4mL(150mL三角瓶)。更多还原

【Abstract】 One strain which could use pyrene as sole carbon source was isolated from oil-contaminated soil in Shenfu irrigation area by selective enrichment culture.The strain was identified as Aminobacter ciceronei based on morphological and physio-biochemical characteristics,fatty aid content analysis and homology identification of 16S rDNA sequence.When the initial concentrations of pyrene was 1mg·L-1 in the inorganic medium,the degradation rates of pyrene by B05 in 10 days was 51 %.When the initial concentrations of pyrene was 1mg·kg-1 in soil,the degradation rates of pyrene by B05 in 30 days was 51 %.When the initial concentrations of pyrene was 50mg·L-1 in the medium with ethanolsoil,the degradation rates of pyrene by B05 in 5 days was 25.9%.It was suggested that the optimization of culture condition was:pH 7.29,32.5℃,25.4ml inventory(150 ml flask).更多还原