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非水介质大孔树脂分离纯化虾壳中虾青素

Separation and purification of astaxanthin from shrimp waste by non-aqueous medium macroporous resin

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【作者】 陈志强金杨任璐

【Author】 CHEN Zhi-qiang1,2,JIN Yang1,REN Lu1(1.College of Food Engineering and Biotechnology,Key Laboratory of Food Nutrition andSafety of Ministry of Education,Tianjin University of Science & Technology,Tianjin 300457,China;2.Jianfeng Natural Product R&D Co.Ltd.,Tianjin 300457,China)

【机构】 天津科技大学食品工程与生物技术学院教育部食品营养与安全重点实验室天津市尖峰天然产物研究开发有限公司

【摘要】 通过7种大孔树脂对虾青素的静态吸附容量和解析率等指标的考察,筛选出AB-8大孔吸附树脂,用于分离虾壳中虾青素,同时利用高效液相色谱(HPLC)法测量虾青素的含量。结果表明,AB-8树脂对虾青素的吸附量为(24.17±0.5)mg/g,解吸率为95.2%,最大上样量(每g干树脂)以虾青素计为(23.07±0.2)mg,并确定用8倍量柱床体积的乙酸乙酯为洗脱剂,纯化所得虾青素的纯度为14.73%。

【Abstract】 Seven types of macroporous adsorption resins were screened with respect to the static adsorption capacity and desorption ratio of astaxantin of shrimp waste and the AB-8 type was chosen as the best one.Astaxantin of shrimp waste was analyzed by high performance liquid chromatography.The results showed that the static adsorption quantity reached(24.17±0.5) mg/g,the desorption rate was 95.2%.The maximal adsorption quantity of astaxantin was(23.07±0.2) mg/g dry resin,the determined elution solvent was eight-fold column volume of ethlacetate,and the product purity was 14.73%.

【关键词】 大孔树脂虾壳虾青素吸附
【Key words】 macroporous resinshrimp wasteastaxantinadsorption
【基金】 天津科技大学引进人才科研启动基金资助项目(20060439,0200096)
  • 【文献出处】 生物加工过程 ,Chinese Journal of Bioprocess Engineering , 编辑部邮箱 ,2009年03期
  • 【分类号】TQ914.2
  • 【被引频次】18
  • 【下载频次】357
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