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非水介质大孔树脂分离纯化虾壳中虾青素
Separation and purification of astaxanthin from shrimp waste by non-aqueous medium macroporous resin
【摘要】 通过7种大孔树脂对虾青素的静态吸附容量和解析率等指标的考察,筛选出AB-8大孔吸附树脂,用于分离虾壳中虾青素,同时利用高效液相色谱(HPLC)法测量虾青素的含量。结果表明,AB-8树脂对虾青素的吸附量为(24.17±0.5)mg/g,解吸率为95.2%,最大上样量(每g干树脂)以虾青素计为(23.07±0.2)mg,并确定用8倍量柱床体积的乙酸乙酯为洗脱剂,纯化所得虾青素的纯度为14.73%。
【Abstract】 Seven types of macroporous adsorption resins were screened with respect to the static adsorption capacity and desorption ratio of astaxantin of shrimp waste and the AB-8 type was chosen as the best one.Astaxantin of shrimp waste was analyzed by high performance liquid chromatography.The results showed that the static adsorption quantity reached(24.17±0.5) mg/g,the desorption rate was 95.2%.The maximal adsorption quantity of astaxantin was(23.07±0.2) mg/g dry resin,the determined elution solvent was eight-fold column volume of ethlacetate,and the product purity was 14.73%.
- 【文献出处】 生物加工过程 ,Chinese Journal of Bioprocess Engineering , 编辑部邮箱 ,2009年03期
- 【分类号】TQ914.2
- 【被引频次】18
- 【下载频次】357