【摘要】 建立一种基于纳米金生物条形码技术的二噁英快速筛检方法.利用二噁英诱导激活的芳香烃受体复合物,特异识别以纳米金为报告基团的二噁英反应探针,该探针被释放后进行条形码放大,通过纳米金银染技术增强识别信号,并记录其吸光度值,从而可以简单灵敏地快速筛检二噁英类化合物.在一定的反应时间和浓度范围内(10-14～10-10mol/L),溶液的吸光度值与2,3,7,8-四氯二苯并二噁英(2,3,7,8-tetrachlorodibenzo-p-dioxin,TCDD)浓度之间呈正相关,方法检测限为0.01pmol/L,变异系数为5%～8%.用纳米金生物条形码(Nano-Barcode,nanoparticle-based bio-barcode)方法和现有的生物分析方法(CALUX,chemical-activated luciferase gene expression)分别测定TCDD标准品,并绘制剂量效应曲线.结果表明,本方法灵敏度高,线性范围宽,重复性好.本研究纳米金生物条形码方法的检测灵敏度高于CALUX将近5倍(EC50分别为4×10-12mol/L和2×10-11mol/L),检测限较CALUX降低了10倍(检测限分别为1×10-14mol/L和1×10-13mol/L),变异系数分别为5%～8%和15%～30%.更多还原

【Abstract】 We have established a nanoparticle-based bio-barcode method for the prescreening of dioxins.The formation of AhR-Arnt complex triggered by dioxin resulted in a high-affinity to DRE.The DRE probes were completely released as bio-barcodes and amplified by nanoparticle based silver enhancement,then the absorbance of TCDD（2,3,7,8-tetrachlorodibenzo-p-dioxin）were recorded as quantitative signals.The dose-dependency of the assay range between 10-14 mol/L to 10-10 mol/L for soluable products.The TCDD detection limit was 0.01 pmol/L with a coefficient of variation as 5%-8%.Compared with CALUX（chemical-activated luciferase gene expression） bioassay,the sensitivity of our Nano-Barcode method was approximately 5 times higher with EC₅0 of 4×10-12 mol/L,and the detection limit was 10 times lower as 1×10-14 mol/L.In addition,our method exhibited a better reproducibility of CV as 5%-8% compared to 15%-30% of CALUX.更多还原