【摘要】 本研究以光化学固定的方法,将TNF-α/IFN-γ共同偶联到24孔聚苯乙烯基板上,利用JC-1为荧光探针,采用流式细胞术在细胞水平检测共固定化及游离细胞因子诱导人宫颈癌HeLa细胞凋亡中线粒体膜电位(△Ψm)变化,进一步探讨共固定化细胞因子与游离细胞因子诱导人宫颈癌HeLa细胞凋亡的作用机制。实验结果显示,共固定化细胞因子诱导HeLa细胞凋亡不存在剂量效应关系,低剂量(20ng/ml)光固定化细胞因子在6d内能使HeLa细胞线粒体膜电位持续下降,具有长效活性,并表明该凋亡过程与线粒体途径密切相关。更多还原

【Abstract】 This study inquired into the mechanisms of co-immobilized cytokines and free cytokines-induced apoptosis on HeLa cells. With the use of photochemical fixed method,TNF-α/IFN-γ were co-immobilized on a 24-well polystyrene culture plate. HeLa cells were stained with fluorescent probe JC-1 to detect the changes of mitochondrial membrane potential (△Ψm),and then were examined by flow cytometry. The results showed that co-immobilized cytokines could induce the apoptosis of HeLa cells in a dose-independent manner. When treated with low-dose of co-immobilized cytokines (20ng/ml),the mitochondrial membrane potential (△Ψm) of HeLa cells continually decreased in 6 days. These indicate that low dose co-immobilized cytokines have a long term of apoptosis-inducing effect on HeLa cells. We assume that there is close relationship between the mitochondrial membrane potential decrease and the apoptosis of HeLa cells.更多还原