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基于微卫星标记的5个尼罗罗非鱼品系的遗传多样性分析
Genetic diversity based on microsatellite markers in five Nile tilapia strains
【摘要】 为了对生产上应用的5个尼罗罗非鱼(Oreochromis niloticus)品系的遗传背景进行本底调查,以期为育种工作提供基础数据,本文用19对微卫星引物对5个尼罗罗非鱼品系(苏丹品系、台湾品系、吉富品系、超雄品系Ⅰ和超雄品系Ⅱ)进行了遗传多样性分析,计算并统计了等位基因数、多态信息含量(PIC)、杂合度、遗传相似性系数、遗传距离等参数。结果表明19个微卫星位点在5个罗非鱼品系中共检测到113个等位基因,平均期望杂合度在0.578-0.692之间,平均多态信息含量在0.473-0.628之间。5个尼罗罗非鱼品系有较丰富的遗传多样性,而超雄品系Ⅰ的遗传多样性相对较为贫乏。
【Abstract】 To provide background on the genetics of cultured Nile tilapia(Oreochromis niloticus)strains and basic information for breeding,we surveyed five Nile tilapia strains(Sudan,Taiwan,GIFT,SupermaleⅠand Supermale Ⅱ)using 19 microsatellite loci to estimate the genetic variation.A total of 113 alleles were de-tected.Mean expected heterozygosity(He)ranged between 0.578 and 0.692,and mean polymorphism infor-mation content(PIC)ranged between 0.473 and 0.628,indicating that these five Nile tilapia strains were ge-netically diverse.Of the five strains,the supermaleⅠhad less genetic variation compared with other strains.
【Key words】 Oreochromis niloticus; genetic breeding; genetic variation; supermale strains;
- 【文献出处】 生物多样性 ,Biodiversity Science , 编辑部邮箱 ,2009年01期
- 【分类号】S917.4;Q953
- 【被引频次】21
- 【下载频次】376