【摘要】 目的:探讨白细胞介素-1(interleukin-1,IL-1),尤其是白细胞介素-1α(IL-1α)和白细胞介素-1受体拮抗剂(IL-1RA)基因多态性与东北地区汉族人群多发性硬化(multiple sclerosis,MS)的关系。方法:多聚酶链反应(PCR)进行IL-1α和IL-1RA基因多态性检测。结果:IL-1α和IL-1RA基因型分布及等位基因频率在MS和对照组间无显著性差异(P>0.05)。结论:IL-1α和IL-1RA基因多态性与MS易感性无相关性。更多还原

【Abstract】 Objective:To explore the relationship between interleukin-1,especially interleukin-1α(IL-1α) and interleukin-1 receptor antagonist(IL-1RA) gene polymorphism and the multiple sclerosis(MS) patients in the Northeast of China. Methods:DNA of white blood cells from 54 subjects with MS and 60 healthy controls was extracted. Polymorphisms in IL-1α and IL-1RA were analyzed with PCR in order to find the difference of the genotype distribution and the allele frequency between the MS patients and the healthy controls. Results:The genotype(CC,CT,TT) distribution of IL-1 α was 40.7%,24.1%,and35.2% in patients with MS and 43.4%,23.3%,and 33.3% in controls(P>0.05) ;while for the IL-1RA(I/I,I/II,II/II,I/IV) was81.5%,7.4%,5.6%,5.5% in MS patients and 80.0%,8.3%,5.0%,6.7% in controls(P>0.05) . The allele frequency of IL-1α in patients with MS were C 57.4%,T42.6% and 60.0%,40.0% in controls;while for the IL-1RA(I,II,IV) were 85.2%,9.3%,5.5% in MS patients and 83.3%,10.0%,6.7% in controls.(P>0.05) . Conclusion:The IL-1α and IL-1RA polymorphisms were not significantly associated with the susceptibility of MS.更多还原