【摘要】 目的:观察高温对人胃癌耐药细胞多药耐药性的逆转作用。方法:对人胃癌耐药细胞株SGC7901/ADM予高温43℃处理,用MTT试验检测高温对ADM、5-FU、DDP、TAX作用下细胞生存率和IC50,并以人胃癌敏感细胞株SGC7901为对照。结果:实验发现人胃癌耐药株SGC7901/ADM细胞除了对诱导耐药的ADM耐受,对CDDP、5-FU、TAX也有交叉耐药。加温至43℃,耐药株SGC7901/ADM细胞的耐药指数明显下降,而且对ADM组、CDDP组、TAX组人胃癌耐药株SGC7901/ADM细胞耐药逆转倍数分别为3.77、2.24、6.25,但对5-FU组SGC7901/ADM细胞的耐药逆转指数较低,为1.11。结论:高温可以增加耐药株SGC7901/ADM细胞对化疗药物ADM、DDP、TAX的敏感性,一定程度逆转细胞的多药耐药性。更多还原

【Abstract】 Objective:To investigate the reversal effect of hyperthermia on multidrug resistance of SGC7901/ADM cell line. Methods:Drug resistant human gastric cancer cell line SGC7901/ADM and sensitive human gastric cancer cell line SGC7901 has been selected.MTT test was employed to detect the cell survival rate and IC50 in the different temperatures and different drugs.Results:It was found that drug resistant human gastric cancer cell line SGC7901/ADM showed not only resistance to the inductive ADM but also cross resistance to CDDP,5-FU and TAX.High temperature of 43℃could lower the survival rate of SGC7901/ADM cell and resistance index (RI)significantly.Reversal multiple to chemical drugs like ADM,CDDP,TAX,5-FU is respectively 3.77,2.24,6.25 and 1.11. Conclusions:43℃hyperthermia could effectively enhance the sensitivity of SGC7901/ADM cell to chemical drugs like ADM,CDDP, TAX and partially reverse the multidrug resistance.It is suggested that hyperthermia could be used to overcome multidrug resistance.更多还原