【摘要】 目的:检测胃癌以及相应癌旁组织中细胞凋亡与c-myc及Ki-67蛋白的表达,探讨它们在胃癌发生发展中的作用。方法:选取58例胃癌和相应癌旁组织作为研究对象,采用脱氧核苷酸移换酶(TdT)介导的dUTP切口末端标记方法(TUNEL)检测细胞凋亡指数(AI),免疫组化技术检测c-myc及Ki-67蛋白的表达。结果:胃癌组织及癌旁组织中细胞凋亡指数(AI)平均值分别为0.969±0.413和2.033±0.600,统计学分析表明:两组间AI平均值差别有显著性(t=11.125,P<0.01)。58例胃癌及癌旁组织中c-myc蛋白的阳性率分别为55.93%(33/58)和36.21%(21/58),两组比较有显著性差异(x2=5.042,P<0.05);58例胃癌(GC)、16例伴癌前病变癌旁粘膜(MAWPL)、42例不伴癌前病变癌旁粘膜(MAWOPL)组织中Ki-67指数分别为35.716±15.091、31.817±10.332和12.441±10.912,统计学分析结果显示胃癌和MAWPL组织中Ki-67指数明显高于MAWOPL组织,差异有显著性(P<0.01)。58例胃癌组织中c-myc阳性组KI平均值明显高于c-myc阴性组(t=3.364,P<0.01)。结论:胃癌组织中存在c-myc基因的异常表达和Ki-67蛋白的过表达。c-myc阳性胃癌的KI平均值明显高于c-myc阴性胃癌,提示c-myc表达可以诱导肿瘤细胞活跃增殖。更多还原

【Abstract】 Objective: To explore the changed regularity of c-myc and Ki-67 protein abnormality in the oncogenic pathway of gastric carcinoma. Methods: The apoptotic cells was tested by terminal deoxynucleotidy1 transferase (TdT)-mediated dUTP-biotin nick end labelling(TUNEL),and the expression of c-myc and Ki-67 protein were tested by immunohistochemistry in 58 GCs with matched clinicopathological data. Results: Apoptotic index of gastric carcinomas and paracarcinomas were 0.969±0.413 and 2.033±0.600,respectively. The AI had a significant difference between gastric carcinomas and paracarcinomas(t=11.125,P<0.01). The expression of c-myc protein was detectded in 33 of 58 (55.39%) gastric carcinomas and in 21 of 58 (36.21%) adjacent tissues. There was significant difference between the two groups (x2=5.042,P <0.05). The Ki-67 index was 35.716±15.091 in gastric carcinomas,31.817±10.332 in paracarcino- mas with preencerous lesion and 12.441±10.912 paracarcinomas without precancerous lesion. The Ki-67 index in gastric carcinomas and paracarcinomas with precancerous lesion was higher than in paracarcinomas without precancerous lesion and there was significant difference between them (t=3.364,P<0.01). Conclusion: The abnormal expression of c-myc and Ki-67 protein is related with the oncogenesis of gastric carcinoma.更多还原