【摘要】 目的:研究经通络方药作用后的脑微血管内皮细胞条件培养液对MIP-1β诱导的大鼠小胶质细胞迁移的影响,以及由MIP-1β在小胶质细胞上的受体-CCR5介导细胞信号转导通路的调控作用。方法:将通络方药作用后的大鼠脑微血管内皮细胞条件培养液加入到由5nMMIP-1β刺激6h后的原代大鼠小胶质细胞中,利用Transwell细胞迁移系统来观察小胶质细胞迁移,用Western blot法检测小胶质细胞CCR5,p-p38,p-JNK表达情况。结果:脑微血管内皮细胞条件培养液能够显著减少小胶质细胞迁移到Transwell下层的细胞数量(P<0.01),降低小胶质细胞上MIP-1β的受体CCR5表达,同时抑制了其下游信号蛋白p38和JNK的磷酸化。结论:通络方药作用后的脑微血管内皮细胞条件培养液可抑制由MIP-1β诱导的大鼠小胶质细胞迁移,其作用发挥可能是通过抑制MIP-1β的受体CCR5表达,降低了其下游通路上p38和JNK蛋白磷酸化程度实现。更多还原

【Abstract】 Objective:We focused our study on the effect of cerebral micro-vascular endothelial cells conditioned medium on microglia migration induced by MIP-1β and its regulation on concerned protein of cell signaling.Method:Add cerebral micro-vascular endothelial cells conditioned medium with Tongluo Jiunao Injection in primary cultured rat microglia stimulated by 5nM MIP-1β for 6h, then observe the microglia migration through Transwell system, and then detect the protein expression of CCR5, p-p38 and p-JNK on microglia.Result:Cerebral micro-vascular endothelial cells conditioned medium reduced the count of microglia that migrated to the lower side of the filter membrane significantly and inhibited the expression of downstream protein p-p38 and p-JNK.Conclusion:The cerebral micro-vascular endothelial cells conditioned medium with Tongluo Jiunao Injection could significantly inhibit microglia migration induced by MIP-1β, and the mechanism of this effect may be the inhibition to the expression of receptor CCR5 and the phosphorylation of p38 MAPK and SAPK/JNK.更多还原