【摘要】 目的:探讨一种理想的急性肝衰竭(AHF)模型建立方法。方法:36只实验兔随机分为3组:①改良药物手术诱导组(A组,n=12),先用D-氨基半乳糖(D-Galn)和脂多糖(LPS)腹腔注射,同时加用乳果糖,注射后2h以氟烷作为麻醉剂,切除约50%肝脏组织,术中经肝静脉注入5%葡萄糖氯化钠溶液10ml/kg体重;②传统手术诱导组(B组,n=12),切除约95%肝脏组织,术中不行肝静脉穿刺注射5%葡萄糖氯化钠溶液;③药物诱导组(C组,n=12),用D-氨基半乳糖(D-Galn)和脂多糖(LPS)一次性腹腔注射。比较建模死亡率、建模后24h兔存活率、血谷丙转氨酶(ALT)、血氨(NH3)、总胆红素(TB)和血糖(BS)。结果:B组手术死亡率高于A组死亡率(41.77%vs0%),A组、B组兔建模成功后24h存活率及C组兔建模成功后72h存活率分别为0%,0%,25%,A组ALT和NH3水平显著高于C组(P<0.05),TB和BG水平低于C组,但差异无显著性。结论:通过改进的50%肝切除术可建立较理想的兔AHF模型,以氟烷作为麻醉剂,药物诱导注射同时加用乳果糖,术中经中叶肝静脉注入5%葡萄糖氯化钠溶液可减少手术死亡率。更多还原

【Abstract】 Objective: To explore an ideal method in establishing a rabbit acute hepatic failure ( AHF ) model. Methods: 36 rabbits were randomly divided into three groups. In modified drug and operation induced group (group A, n=12), a rabbit AHF model was established by D-galactosamine (D-Galn) and lipopolysaccharide (LPS) injected into the abdominal cavity once and then 50% of liver was resected. In the operation, 10ml 5% glucose saline solution per kilogram of body weight was injected into the median lobe hepatic vein of the rabbit. In traditional operation induced group (group B, n=12), 95% of liver was resected, but no glucose saline solution was injected. In drug induced group (group C, n=12), AHF model was established by D-galactosamine (D-Galn)and lipopolysaccharide (LPS) injected into the abdominal cavity once. Operative mortality and 24h survival rabbit after establishment of models were observed. ALT, total bilirubin (TB), NH3 and blood sugar (BG) were monitored. Results: The operation mortality in group B was higher than that of group A (41.7% vs 0%). Survival rabbit was 0%, 0% and 25% in group A, group B and group C later respectively. ALT and NH3 in group A were significantly higher than those of group C (P<0.05). TBIL and BG levels in group A were lower than those in group C, but the difference was not significant. Conclusions: The AHF model established by D-galactosamine (D-Galn) and lipopolysaccharide (LPS) injected into the abdominal cavity once and then 50% hepatectomy resected is an ideal method.更多还原