【摘要】 目的:探讨脊髓损伤(Spinal cord injury,SCI)后,诱生型一氧化氮酶(Inducible nitric oxide synthase,INOS)与内皮素(En-dothelin,ET)的表达情况。方法:Wistar大鼠36只,随机分成3组,为正常对照组、轻(中)度损伤组和重度损伤组。大鼠在脊髓损伤后14天处死。应用S-P免疫组化法检测INOS和ET表达情况。结果:正常组几乎未见INOS和ET表达,在损伤14天后,轻(中)度脊髓损伤和重度脊髓损伤中的INOS的阳性率分别为14.8%和59.3%,两组有显著性差异(P=0.004)。ET的阳性率分别为11.1%和51.9%,两组有显著性差异(P=0.013)。结论:INOS与ET参与了大鼠脊髓损伤过程,并且与损伤程度相关。更多还原

【Abstract】 Objective:To study the expression of inducible nitric oxide synthase(INOS) and endothelin(ET) after spinal cord inju-ry in rats.Methods:36 adult Wistar rats were randomly divided into normal,mild(moderate) and severe injury groups.The rats were killed at 14 days after spinal cord injury.Expression of INOS and ET was determined with S-P immunohistochemical methods.Results:There were no INOS and ET expression in cells of normal group.14 days after spinal cord injury,the INOS(ET) levels in mild(moderate) and severe groups were 14.8%(11.1%) and 59.3%(51.9%),respectively;there were significant difference(P=0.004;P=0.013).Conclu-sions:INOS and ET take part in the process of rats’ spinal cord injury.The expression of INOS and ET showed increased tendency with the spinal cord injury degree.更多还原