【摘要】 利用BIOLOG YT、FF微孔板分别考察了4个真菌群落代谢活性及群落间的代谢相似性,并与聚合酶链式反应-变性梯度凝胶电泳(PCR-DGGE)结构相似性分析对比试图探讨代谢相似性与结构相似性的内在联系,探讨了超低温冻存法作为样品保存手段对真菌群落特征BIOLOG分析结果的影响。结果表明:两种微孔板所反映的代谢相似性聚类分析结果完全不同,FF板所反映的代谢相似性聚类分析规律与PCR-DGGE提供的种群结构聚类分析规律一致;超低温冻存处理影响显著影响BIOLOGYT代谢活性(P=0.023)和BIOLOG FF多样性指数(H’)(P=0.041),但对两种微孔板所反映的其它指数如代谢活性、丰富度指数(S)、多样性指数(H’)分析结果均无显著性影响(P>0.05)。更多还原

【Abstract】 BIOLOG YT and FF microplates were used in this study to investigate the metabolic activities and similarity of 4 fungal communities in environmental samples.Subsequently,the BIOLOG metabolic similarity expressed by these two microplates was linked to structural similarity expressed by Polymerase Chain Reaction-Denaturing Gradient Gel Electrophoresis(PCR-DGGE).In addition,the effect of cryopreservation on BIOLOG analytic results,including metabolic activities,richness index and diversity index,was also discussed.The following conclusions have been drawn:(1) BIOLOG FF microplate expressed a metabolic similarity relation among 4 fungal communities absolutely different from that by BIOLOG YT microplate.(2) The BIOLOG FF metabolic similarity of 4 fungal communities nearly corresponded to the PCR-DGGE structural similarity.(3) The cryopreservation had no significant influence on BIOLOG richness index,BIOLOG FF metabolic activities and BIOOG YT diversity index(P > 0.05).更多还原