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福建东张水库陆封型香鱼的遗传变异

Genetic variation of lacustrine Plecoglossus altivelis population in Dongzhang Reservoir, Fujiang Province

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【作者】 鲁延付乐小亮赵爽陈泉梅章群

【Author】 LU Yan-fu, YUE Xiao-liang, ZHAO Shuang, CHEN Quan-mei, ZHANG QunResearch Center of Hydrobiology, Jinan University, Guangzhou 510632, China

【机构】 暨南大学水生生物研究所

【摘要】 测定了福建东张水库18尾陆封型香鱼的ND4-tRNASer基因533 bp序列,发现2个可变位点和2个简约信息位点,共检测到4种单倍型。基于Kimura 2-parameter模型的遗传距离在0.001 9~0.003 9之间,在NJ系统树上无明显谱系结构;单倍型多样性(Hd)和核苷酸多样性(Pi)分别为0.627和0.001 37,平均核苷酸差异数为0.732,远低于两侧洄游型日本香鱼指名亚种(Hd为0.793,Pi为0.002 94),与被列为IUCN濒危物种名录的日本琉球香鱼亚种(Hd=0.519,Pi=0.001 11)相当。东张水库香鱼遗传多样性如此贫乏到底是历史原因还是近期陆封的结果,仍需进一步研究。目前亟待采取有效措施保护东张水库陆封香鱼。

【Abstract】 Five hundred and thirty three bp sequence of partial mitochondrial ND4-tRNASer gene of 18 individuals of lacustrine Plecoglossus altivelis population from Dongzhang Reservoir, Fujiang Province was determined, and 4 haplotypes, 2 variable sites and 2 parsimony-informative sites were detected. Genetic distance based on Kimura 2-parameter model among 4 haplotypes ranged from 0.001 9 to 0.003 9. No obvious genealogical clusters were found in neighbor-joining tree. Haplotype diversity(Hd) and nucleotide diversity(Pi) were 0.627 and 0.001 37 respectively with average nucleotide difference 0.732 in the population, which were much lower than in Plecoglossus altivelis altivelis in Japan(Hd=0.793, Pi=0.002 94), but similar as compared with Plecoglossus altivelis ryukyuensis, an endangered subspecies listed in IUCN(Hd=0.519, Pi=0.001 11). Further studies are still needed to make clear the reasons responsible for such poor genetic diversities in Dongzhang reservoir populations; meanwhile urgent measures should be taken to conserve the valuable fishes.

【关键词】 香鱼ND4-tRNASer遗传变异
【Key words】 Plecoglossus altivelisND4-tRNASergenetic variation
【基金】 国家自然科学基金(30770415);留学归国人员科研启动基金(2005)资助项目
  • 【文献出处】 生态科学 ,Ecological Science , 编辑部邮箱 ,2009年06期
  • 【分类号】S917.4
  • 【被引频次】5
  • 【下载频次】30
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