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蒽酮硫酸法与苯酚硫酸法测定枸杞子中多糖含量的比较

Comparison of methods in determination of polysaccharide in Lycium barbarum L.

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【作者】 刘晓涵陈永刚林励庄满贤方晓娟

【Author】 LIU Xiao-han, CHEN Yong-gang, LIN Li, ZHUANG Man-xian, FANG Xiao-juan (College of Chinese Traditional Medicne, Guangzhou University of Chinese Medicine, Guangzhou 510006)

【机构】 广州中医药大学中药学院

【摘要】 目的:比较蒽酮-硫酸法与苯酚-硫酸法测定枸杞子中多糖含量的差异。方法:分别采用上述两种方法利用紫外-可见分光光度计测定枸杞子中多糖的含量。结果:蒽酮-硫酸法测定结果高于苯酚-硫酸法,其差异有统计学意义。结论:苯酚-硫酸法测定结果更为准确合理,可考虑作为枸杞子中多糖含量测定的首选方法。

【Abstract】 Objective: To compare difference in determination content between phenol -vitriolic colorimetry method and anthrone sulphuric acid colrimetry method for polysaccharides in Lycium barbarum. Methods: Above two methods were employed to determine content of polysaccharides by UV spectrophotography. Results: The determination value of anthrone-sulphuric acid colorimetry method is high than the phenol-vitriolic colorimetry method, and its values dissimilarity have statistical significance between two methods.Conclusion: The result of phenol-vitriolic colorimetry method is more reliable and accurate, it can be considered as the optimum assay method for polysaccharides in Lycium barbarum.

  • 【文献出处】 食品科技 ,Food Science and Technology , 编辑部邮箱 ,2009年09期
  • 【分类号】R284.1
  • 【被引频次】262
  • 【下载频次】6754
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