【摘要】 研究南瓜多糖硫酸酯化修饰与结构稳定性之间的关系。采用三氧化硫-吡啶法对南瓜多糖PL2-1进行硫酸酯化修饰,取代度为0.19。通过气相色谱、红外光谱、高碘酸氧化、Smith降解及甲基化等方法对南瓜多糖PL2-1进行结构初步研究。实验结果表明,PL2-1为半乳聚糖,主链和侧链主要以1→4位键合,少量以1→3,6位键合的半乳糖构成主链分枝点,间隔排布于1→4位键之间,13.0%的半乳糖位于非还原末端。更多还原

【Abstract】 This study focused on the relationship of sulfation and structural stability of polysaccharides from pumpkin pulp.Polysaccharide PL2-1 was chemically modified by method of sulfur trioxide-pyridine(SO·3Py) complex as a reagent,and DS of SPL2-1 was 0.19.The primary structure of PL2-1 was elucidated based on GC,IR spectra,periodate oxidation,Smith degradation and methylation analysis.PL2-1 was found to be a galactan,whose main chain consists of(1→4)-linked galactose,and a few of 1→3,6 linkage,as branched points,arranged in the interval 1→4 linkage between.Some of them were located at the end of unreduced group.更多还原