【摘要】 利用酶解法制备琥珀酸产生菌的孢子原生质体,对其进行紫外线与He-Ne激光的复合诱变,筛选到了一株突变株MS-23,其琥珀酸产量为1.27g/L,是出发菌株产酸量的20.16倍。将此菌株连续传代5次,具有较好的遗传稳定性。更多还原

【Abstract】 The protoplasts from spores of a strain producing succinic acid were prepared by enzyme and irradiated by both UV and He -Ne laser. After the compound mutation, a high-yielding strain MS-23 was obtained, whose yield of succinic acid is 1.27 g/L, about 20.16 times of its parent. After being propagated for five generations, the mutation strain MS-23 shows a more stable heredity.更多还原