【摘要】 以白色和橘红色块根的甘薯为试材,探讨高效液相色谱(HPLC)检测块根提取物中β-胡萝卜素的方法,同时分析了两种颜色块根样品中β-胡萝卜素的含量。结果发现:用正己烷/丙酮/乙醇(6/3/1)提取块根样品,HPLC流动相为甲醇与甲醇/异丙醇(40/60)梯度洗脱系统时,样品中的β-胡萝卜素得到很好的分离。β-胡萝卜素在0.002～0.05mg/mL范围内与色谱峰面积有很好的线性关(R2=0.9992)。平均回收率为104.67%,精密度RSD为1.14%。该法测定样品,准确可信,重复性好。此外通过HPLC定量分析,就β-胡萝卜素而言,橘红色块根甘薯比白色块根品种更具营养价值。更多还原

【Abstract】 In the present study, the white and orange-red roots of sweet potato were chosen as materials, and the HPLC analysis method on β-carotene in root extracts was developed. In addition, the quantitative analysis on β-carotene in two kinds of roots was also performed in this paper. The results showed that β-carotene could be extracted from sweet potato roots using hexane/acetone/ethanol(6/3/1). It could be identified on HPLC using gra- dient mobile phase methanol and methanol/isopropanol (40/60). The liner range of β-carotene was 0.002～0.05 mg/ mL, the average recoveries and RSD were 104.67% and 1.14%, respectively. It is an accurate and repeatable method for determining β-carotene in sweet potato roots. By quantitative analysis on HPLC, orange-red roots with β-carotene provided more nutritional benefits than white ones without β-carotene.更多还原