【摘要】 支持电极是影响免疫传感器发展的关键技术问题之一。通过近5年免疫传感器的研究,对支持电极进行了系列选择和研究改进工作。在研究中选用过3种固体单电极,单通道丝网印刷碳电极,改进制备的四通道、五通道和八通道丝网印刷碳电极。采用激光扫描共聚焦显微镜(LSCM)进行形态学表征,通过循环伏安法(CV)进行电化学表征,评估电极的性能,初步选定四通道丝网印刷碳电极(4-SPCE)为支持电极。用物理吸附法制备辣根过氧化物酶(HRP)酶电极,以检验选定的4-SPCE的应用性能。结果显示,改进制备的4-SPCE的应用性能最好,因此选用该4-SPCE用作电化学免疫传感器研究中的支持电极。更多还原

【Abstract】 Supporting electrode was one of the key technologies in the development of immunosensor. Through the research on immunosensors of the last five years, supporting electrode had been chosen and improved; three kinds of solid electrodes, single-channel screen-printed carbon electrode (SPCE), improved four-channel, five-channel and eight-channel SPCE, were selected. The electrodes were characterized by laser scanning confocal microscope (LSCM) and electrochemical method based on cyclic voltammetry (CV). Four-channel screen-printed carbon electrode (4-SPCE) was selected as preliminary supporting electrode. Horseradish peroxidase (HRP) electrode was prepared by physical adsorption. The enzyme electrode was used to test the performance of the 4-SPCE, the result of experiment indicated that improved 4-SPCE had the most excellent application performance. Therefore, the 4 -SPCE was used as supporting electrode in developing electrochemical immunosensor.更多还原