【摘要】 利用PCR技术扩增我国3个不同海域(渤海湾、东海的舟山海域、南海的广东沿海)银鲳(Pampus argenteus)线粒体COⅠ的基因片段,得到长度为604bp的片段,比较分析了3个不同地区48尾银鲳线粒体COⅠ基因序列的变异和遗传结构。结果显示,48条序列共检测出多态性位点30个,其中简约信息位点6个,各群体内的多态性位点分别为渤海8个、舟山26个、广东1个;48个个体具有18个单倍型,单倍型多样性和核苷酸多样性分别为0.6622和0.0028。AMOVA分析结果表明,群体间的遗传变异占总遗传变异的-1.03%,而101.03%的遗传变异源于群体内,3群体总的遗传分化指数(FST)为-0.0103(P>0.05)。此外,研究结果还显示,群体间遗传分化指数与遗传距离均非常低。分析得出:基于银鲳线粒体COⅠ基因的序列分析,单倍型多样性以东海群体最高(0.8009)、渤海群体其次(0.7000)、南海群体最低(0.2000),而3群体核苷酸多样性则均较低(<0.005);分子变异分析未检测出我国3个野生银鲳群体间存在遗传分化现象。更多还原

【Abstract】 The partial mitochondrial COⅠ sequences of 48 silver pomfret(Pampus argenteus) from three areas [Bohai Sea(B),East China Sea of Zhoushan(E),South China Sea of Guangdong(S)] were amplified by PCR technique to analyze their sequence variation and genetic diversity.604 bp COⅠ gene fragments were obtained.A total of 30 polymorphic sites were detected,including 6 parsim-informative sites.The number of polymorphic sites was 8 for the population B,26 for the population E and 1 for the population S,respectively.There were 18 haplotypes in 48 COⅠ gene sequences.The average haplotype diversity and nucleotide diversity of the three populations were 0.662 2 and 0.002 8,respectively.Analysis of AMOVA indicated that large COⅠgene differentiation(101.03%) occurred within populations,and-1.03% occurred among populations.The total genetic differentiation index(FST) of the three populations was-0.010 3(P>0.05).Additionally,both the FST and k-2-p distances within populations were very low.The present results suggested that the highest haplotype diversity was in population E(0.800 9),the middle was in population B(0.700 0) and the lowest was in population S(0.200 0).The nucleotide diversities in three populations were very low(< 0.005).There was no genetic differentiation in different wild stocks of silver pomfret based on mitochondrial DNA COⅠgene sequences.更多还原