【摘要】 为研究甜菜红色素主要抗氧化成分,采用Sephadex LH-20分离甜菜红色素,收集红色部分和黄色部分,用HPLC法检验分离效果。采用ABTS、DPPH、FRAP3种方法评价甜菜红色素各部分的抗氧化性。结果表明:红色部分与甜菜红色素差异不显著(P>0.05),说明红色部分与甜菜红色素抗氧化能力接近;黄色部分与甜菜红色素差异显著(P<0.05),说明黄色部分的抗氧化能力不及甜菜红色素。表明甜菜红色素主要抗氧化成分为红色部分。更多还原

【Abstract】 Inorder to evaluate the antioxidant activity of the main composition of betalain,the betalain were transferred to Sephadex LH-20 column and separated by elution ultrafiltered wanter adjusted to pH 5-6 with formic acid.Different fractions(red-violet betacyanins and yellow betaxanthins) were collected and then freezedryed.The final purification identification were carried out by high-performance liquid chromatography(HPLC) with different chromatographic conditions.ABTS free radical-scavenging assay,DPPH free radical-scavenging assay and FRAP assay were adopted.Result:The main antioxidant composition of betalain were the red-violet betachyanins.更多还原