【摘要】 为评价中国出口花生企业的黄曲霉毒素检测能力,组织了出口花生企业实验室黄曲霉毒素检测能力验证研究。依据ISO13528:2005、国际能力验证活动的统一规则(IUPAC)以及CNAS(中国合格评定国家认可委员会)规定的程序,对本次能力验证活动进行了实施和评价。参加实验室采用的方法有高效液相色谱法和荧光光度计法。测试样品采用天然黄曲霉毒素污染的花生酱,采用Ss≤0.3σP检验法对待测样品进行了均匀性测试,采用两独立样本均数的t检验法,对样品进行了稳定性测试。采用Z比分数评定各参加实验室的测试结果。各参加实验室提交结果的Robust均数作为测试样品黄曲霉毒素含量的指定值xa。依据Horwitz方程的修正公式σP=0.22c计算能力验证的标准偏差σP。在102个参加实验室中,93家实验室结果"满意",6家实验室结果"可疑",3家实验室结果"不满意"。更多还原

【Abstract】 This assessment program was established by Shandong Imported and Exported Inspection and Quarantine Bureau of China (SDCIQ) to evaluate the aflatoxin-testing capacity of a cross-section of Peanut Export Industry. The assessment was performed according to ISO 13528: 2005 and the International Harmonized Protocol for Proficiency Testing. The 102 industries were involved in the study. The analytic samples for this scheme were prepared from naturally contaminated peanut butter. The contents of aflatoxin were analyzed by high performance liquid chromatography and fluorometry. The Ss≤0.3σP test was used to evaluate the homogeneity of the test samples and the sample stability was confirmed with a t-test. The performance of each laboratory was designated by a z-score that was calculated using robust statistics. The robust mean of the participants results in this study was nearly coincident with the median. A modified Horwitz equation was used to analyze the standard deviation. 93 samples were observed as satisfactory, 6 as questionable and 3 as unsatisfactory. Samples reported to be questionable or unsatisfactory were further re-evaluated in a second inter-laboratory comparison.更多还原