【摘要】 目的:探讨AB-8大孔树脂对槐花中总黄酮静态和动态的吸附性能。方法:用微波辅助法得到粗提液,利用分光光度法测定样品中黄酮含量,考察吸附剂用量、粗提液浓度、pH值、吸附时间等条件对吸附结果的影响。结果:AB-8树脂对槐花中总黄酮具有较好的吸附性能。静态吸附最佳条件:树脂与提取液比为1:20(g/ml)、25℃、pH5～6、粗提液中的黄酮含量0.10～0.17mg/ml、恒温振荡70min、70%乙醇洗脱,在此条件下,黄酮总回收率为71.94%;动态吸附的最佳条件为:提取液上样质量浓度0.045～0.070mg/ml、pH5、以0.2ml/min的流速通过径高比为1:8的层析柱,70%的乙醇4BV洗脱,在此条件下,黄酮总回收率为39.32%。结论:AB-8树脂较适于分离纯化槐花总黄酮。更多还原

【Abstract】 This work studied static and dynamic adsorption and desorption of total flavonoids prepared from pagoda flowers by microwave-assisted extraction on macropurous resin AB-8.Results showed that AB-8 resin had good adsorption capacity to pagoda flower flavonoids;the optimum static adsorption and desoprtion conditions were as follows:adsorption on a shaking table for 80 min at 25 ℃with a ratio of resin to sample 1:20(g/ml), at pH 5-6, sample concentration 0.10-0.17 mg/ml and then desorption with 70% aqueous ethanol as eluent, and under such conditions, the recovery of flavonoids was 71.94%;a recovery of total flavonoids of 39.32% was obtained by the following optimum dynamic adsorption and desoption conditions of sample concentration 0.045-0.070 mg/ml, pH 5, ratio of column diameter to length 1:8, flow rate 0.2 ml/min and 70% aqueous ethanol as eluent.更多还原