【摘要】 本实验以氨基氮含量为指标,采用正交试验考察底物浓度、酶浓度、温度、时间和pH值对氨基氮含量的影响。结果表明:选择中性蛋白酶水解,仔乌肽的最佳酶解条件为料水比1:5(m/m)、加酶量900U/g底物、pH7.0、温度50℃、时间4.0h,此条件下酶解仔乌肽的氨基氮含量为7.34mg/g。更多还原

【Abstract】 In order to optimize enzymolysis process of Loligo japonica protein, effects of protease type, enzyme dose, reaction temperature, reaction time, pH and material/water ratio on amino nitrogen(ANN) content in the hydrolysate were studied using orthogonal array design.Results showed that a maximum ANN content of 7.34 mg/g was achieved by the neutral protease hydrolysis for 4.0 h with a material/water ratio of 1:5(m/m) and an enzyme dose of 900 U/g substrate at 50 ℃, pH 7.0.更多还原