【摘要】 考察pH值和表面活性剂对酿酒酵母生物合成谷胱甘肽(GSH)的影响。设计了一种pH值控制策略,在酿酒酵母HSJB1发酵过程中,当pH1.5时,可使胞内合成的GSH完全分泌到胞外,胞外GSH含量达47.66mg/L。0.1%的十二烷基磺酸钠(SDS)几乎完全抑制细胞的生长,而0.1%的蔗糖酯、吐温80和二甲基亚砜(DMSO)对细胞生长无抑制作用,并且对GSH的胞外分泌也无影响;曲拉通(TritonX-100)对菌体的生长和GSH胞外分泌产生影响,随加入浓度增加,GSH胞外分泌增加,添加TritonX-100时间越早,分泌到胞外的GSH越多。更多还原

【Abstract】 In order to understand effects of pH value on glutathione (GSH) biosynthesis by Saccharomyces cerevisiae,a pH-control strategy was developed and applied in fed-batch production of GSH. Extracellular concentration of gultathione reached 47.66 mg/L when fermentation broth pH value was reduced to 1.5,and intracellular concentration of glutathione was 0.1% The addition of 0.1% SDS into fermentation broth revealed an inhibition effect on yeast growth. In contrast,sucrose ester,Tween-80,DMSO did not exhibit effects on inhibition of yeast growth and secretion of GSH. On the other hand,yeast growth and secretion of GSH were obviously affected by Triton X-100. The higher concentration of Triton X-100 and the earlier addition resulted in more secretion of GSH.更多还原