【摘要】 采用木瓜蛋白酶、中性蛋白酶和Flavourzyme分别水解菜籽粕制取菜籽多肽,研究酶解时添加低浓度Na2SO3对反应速率的影响。结果表明:添加Na2SO3可增加酶对菜籽蛋白的水解速率,但对水解速率的影响程度根据酶的不同而有所不同;木瓜蛋白酶、中性蛋白酶和Flavourzyme添加Na2SO3时水解菜籽蛋白反应60min的水解度分别为未添加Na2SO3的2.5、2.9和1.7倍。凝胶层析结果显示,添加Na2SO3的蛋白水解物的分子量比不加Na2SO3的低。更多还原

【Abstract】 Peptides were prepared by hydrolysis of rapeseed meal by papain, neutrase and flavourzyme, respectively. The effects of sodium sulfite at low concentration on proteolysis rate of rapeseed meal were investigated. The hydrolysis rates of rapeseed protein by the three enzymes were increased by the addition of sodium sulfite to different extents. After the 60 min hydrolysis, the degrees of hydrolysis of rapeseed protein by papain, neutrase and flavourzyme with the addition of sodium sulfite were 2.5, 2.9 and 1.7 times of those in the absence of sodium sulfite, respectively. Size exclusion chromatographic analysis showed that the molecular weights of the rapeseed protein hydrolysates with the addition of sodium sulfite were lower than those without sodium sulfite.更多还原