【摘要】 采用水提分级醇沉法提取南瓜多糖并进行硫酸酯化修饰初步研究。通过DEAE-Sepharose Fast Flow离子交换层析分离纯化得到南瓜多糖PL-2。Sepharose CL-6B凝胶过滤层析和高效液相色谱鉴定PL-2纯度,证明其组分均一,且分子量>2000kD。采用氯磺酸-吡啶法、三氧化硫-吡啶法、氨基磺酸法对南瓜多糖PL-2进行硫酸酯化修饰,BaCl2-明胶比浊法和红外光谱鉴定取代效果,结果表明,三氧化硫-吡啶法的修饰效果相对较好。更多还原

【Abstract】 Crude water-soluble polysaccharides were separated from pumpkin by hot water extraction and gradient ethanol precipitation,and purified/fractionated by DEAE-Sepharose Fast Flow anion-exchange chromatography and a acidic polysaccharide PL-2 was obtained.Sepharose CL-6B gel filtration chromatography and HPLC analysis demonstrated that the component of PL-2 was homogenous and its molecular weight was more than 2000 kD.The polysaccharide was chemically modified by chlorosulfuric acid-pyridine method,sulfur trioxide-pyridine method and sulfamic acid,respectively.The identification of BaCl2-gelatin turbidimetry and infrared spectra revealed that sulfur trioxide-pyridine method displayed the best sulfation effect.更多还原