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用筛选抗分解代谢阻遏突变株法选育谷氨酰胺转胺酶的高产菌株

Screening and Breeding of High-yield Transglutaminase (TGase)-producing Streptomyces hygroscopicus Strain Resistant to 2-deoxy-D-Glucose Repression

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【Author】 XU Xiao-juan,LI Jiang-hua,ZHANG Dong-xu,CHEN Jian,DU Guo-cheng (Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China)

【机构】江南大学生物工程学院,工业生物技术教育部重点实验室；

【摘要】以吸水链霉菌(Streptomyces hygroscopicus)CCTCCM203062为出发菌株,采用N离子注入和紫外诱变处理,得到MTG酶活达到7.19U/ml的高产菌株BB-2。对BB-2再进行紫外诱变,选育出具有较好稳定遗传性的抗2-脱氧-D-葡萄糖的高产突变株DG-1,酶活达到9.21U/ml,比出发菌株提高了118.5%。更多还原

【Abstract】 In the present study, through mutagenizing Streptomyces hygroscopicus CCTCC M203062 with UV and Nimplantation, a mutant strain BB-2 with TGase activity of 7.19 U/ml was obtained. Then a new stain DG-1 resistant to 2-deoxyD-glucose (2-DG) repression was obtained from the mutant strain BB-2 by repeated UV radiation, with good genetic stability and TGase activity of 9.21 U/ml, which was higher 118.5% than that of the original strain CCTCC M203062.更多还原

【关键词】谷氨酰胺转胺酶；吸水链霉菌；紫外诱变； N离子注入； 2-脱氧-D-葡萄糖；
【Key words】 TGase； Streptomyces hygroscopicus； UV mutagenization； N+-implantation； 2-deoxy-D-glucose；

【基金】国家自然科学基金项目(30770055;20776063);江苏省高新技术研究项目(BG2007009);江苏省自然科学基金项目(BK2007018)

【文献出处】食品科学 ,Food Science , 编辑部邮箱 ,2009年09期

【分类号】Q933
【被引频次】10
【下载频次】316

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